The star chaser could do nothing as Bristol De Mai fell in front of him with a circuit to go.
Jefferson's desire to test the waters over three miles has been left untainted, though, and she's keen to get her talented gelding out again as soon as possible.
With few possibilities over the trip left until the Gold Cup, a crack at a shorter distance could be on the cards.
Jefferson said: "He's come back fine, trotted up sound this morning – and his legs are cold, which is the main thing, I suppose.
"It's just one of those things. It wasn't his fault, but that's life.
"It was too early to say what would have happened, but I was very pleased with how he was going and how he was jumping – and Brian was very happy.
"We still don't know if he'll stay three miles. I don't know what we'll do next – but I'm hoping if nothing comes to light to run him sooner rather than later, because he's obviously fit. I don't want to sit on him as it could cause problems.
"We'll consider everything, possibly even back at two miles. I just don't know as yet – we'll just have to see."
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