Lionel Messi is also a doubt for the game on Tuesday as Ernesto Valverde plans to rest several of his big-name players, with the Catalans already assured of a place in the knock-out stage.
Victory for Spurs in the Nou Camp will secure their place in the last-16, but anything less could see Inter Milan snatch second place in Group B.
And Mauricio Pochettino has been given a major boost ahead of the game.
Valverde said: “I don't know if we'll have Lionel Messi out there for two games in three days.
"But Luis Suarez is definitely not going to play.”
Suarez has been nursing a knee injury this term and will sit out the game, despite starting and scoring in the 4-0 win over Espanyol on Saturday.
Messi could also be rested from the starting XI having played the full 90 minutes in each of Barca's last four La Liga fixtures.
Goalkeeper Jasper Cillessen could replace Marc-Andre ter Stegen with a host of other alterations also expected.
The five-time winners face Levante and Celta Vigo before the mid-season break but will not unnecessarily risk players having already won the group.
No side have beaten Barcelona on their home patch in a Champions League group game since Ruben Kazan won 2-1 in October 2009.
But if Inter can overcome PSV Eindhoven in the other game, then nothing less than all three points will be enough for the north Londoners.
Harry Kane and Christian Eriksen were rested from the start against Leicester City as Tottenham cruised to a 2-0 win in the Premier League at the weekend.
Both players can expect to be restored to the team, with Lucas Moura, Erik Lamela and Son Heung-Min scrapping for a starting place.
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