He was set to start work on the upcoming new 007 movie from December in time for it to hit UK cinema screens in October 2019.
But today the official James Bond Twitter account announced: "Michael G. Wilson, Barbara Broccoli and Daniel Craig today announced that due to creative differences Danny Boyle has decided to no longer direct Bond 25."
It's not known whether Boyle's sudden departure will effect the film's release date.
Boyle directed four of the Bond films so far, including Spectre with Daniel Craig.
He has been working with John Hodge (Trainspotting) on a script for the upcoming film.
A previous draft was written Neal Purvis and Robert Wade, who both wrote Casino Royale, Quantum of Solace, Skyfall and Spectre.
Next year's release is expected to be Craig's last in the role.
Bond fans have been speculating who the next actor could be, with Idris Elba and Tom Hardy tipped to be the famous secret agent.
More to follow…
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