The Argentine boss reacted to Jose Maria Giminez's opener by turning to the stands and grabbing his crotch in a bizarre move.
After getting a good handful, Simeone give it all a shake for good measure – clearly thrilled by his side taking the lead in the Spanish capital.
It's the the first time Simeone has performed the celebration against Juventus either, having done it 20 years while playing for Lazio.
And Simeone had more cause for celebration too, after Diego Godin scored his side's second five minutes later.
It could have been 3-0 too, only for Alvaro Morata to have his goal ruled out by VAR earlier in the match for a shove on Giorgio Chiellini.
There was one minor setback on the night though, as Diego Costa ruled himself out of the second-leg through suspension after losing the plot in the first-half.
Despite the massive win, Twitter could only focus on one thing… here's the best of the reaction.
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