The On The Floor hitmaker was performing at her Las Vegas residency show when she took a tumble.
But she showed she's ever the professional as she got straight back up on her feet – with a little bit of help from a member of the audience.
One fan uploaded a video of the incident to Twitter, writing: "This is how you handle a fall like a pro."
Lopez, 49, has recently been house hunting in LA with partner Alex Rodriguez, 43.
She and the former baseball star, who have been dating for over a year, already own a £10million pad in New York.
Recently the couple – nicknamed J-Rod – narrowly avoided a run-in with Jen's ex-boyfriend Casper Smart as they dined out in West Hollywood.
Backup dancer Casper, 31, arrived at swish restaurant Craig's shortly after Jennifer and Alex left.
The mum of two dated Casper on and off between 2011 and 2016, following her divorce from Marc Anthony, 50.
Jennifer welcomed twins Max and Emme, now ten, with singer Marc before their split.
The star also recently shared a series of sizzling snaps as she sunbathed in a very revealing swimsuit.
The first snap proved the chart-topper's behind is far from On The Floor – quite the opposite, in fact – as she stood side-on for the camera.
The second shot was a sultry selfie of the Love Don't Cost A Thing hitmaker as she relaxed on a sun lounger..
JLo playfully captioned the snaps: "Just peachy #tangerinedream #peachesNCream #topnaughty #suitsandfruits #throwingshades #doublebuns. Knot your average peach. To peach their own. Practice what you peach."
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